Believe in What You See.
The tumor microenvironment is complex. Your research doesn’t have to be.
Introducing Bethyl’s IHC PathPlex® Multiplex Antibody Panels. Quickly detect multiple antigens in a single tissue section to gain clear understanding of the microenvironment without compromising samples.
Believe in What You See.
The tumor microenvironment is complex. Your research doesn’t have to be.
Introducing Bethyl’s IHC PathPlex® Multiplex Antibody Panels. Quickly detect multiple antigens in a single tissue section to gain clear understanding of the microenvironment without compromising samples.
With the advancement of immunotherapeutics, the urge to understand the tumor microenvironment has never been more pressing. Molecular histopathology has recently been shifting from single-marker immunohistochemistry towards multiplexed marker detection. Multiplex immunohistochemistry maximizes the amount of data acquired from an individual sample. Recent advances in multiplex immunohistochemistry and multispectral imaging facilitate accurate simultaneous analysis of multiple tissue markers. Clinically, understanding of the tumor microenvironment, and subsequent selection of appropriately-targeted therapies, can be augmented by profiling and localizing immune checkpoint proteins. The applications of multiplex immunohistochemistry are numerous, and span clinical, translational, and basic research applications.
Maximize limited samples
Visualize multiple targets within a single tissue
Preserve tissue architecture
Obtain spatial information and co-expression within the TME
Clear counterstaining with DAPI
Hematoxylin can be obscured by other targets with chromogenic staining
Detect low-level binding sites
Fluorophores have a wider dynamic range and larger linear range than chromogenic substrates
Measure intensity for each target
Fluorescence signals can be overlaid and seen as single or multi-channel
Want to Learn More About Multiplexing?
Read related content:
To serve your multiplex research needs, Bethyl introduces IHC PathPlex® panels to profile the complex tumor microenvironment. Each antibody has been validated for IHC and utilized in a multiplex format by our own scientists via proprietary processes to ensure the highest quality. With over 45 years of experience, Bethyl is recognized as a leading antibody manufacturer trusted by researchers around the world.
Pick any of the three core panels and add one or more antibody of your liking from our immuno-oncology inventory.
IHC PathPlex® Core Panels
T-Cell I/O Panel
CD3
CD8
PD-L1
Lymphocyte Panel
CD3
CD8
CD20
T, B & M Panel
CD3
CD20
CD68
Detection of human CD3 (yellow), CD8 (red), and CD20 (green) in FFPE tonsil by IHC-IF.
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Rabbit anti-CD3e recombinant monoclonal [BL-298-5D12] (A700-016), rabbit anti-CD8a recombinant monoclonal [BLR044F] (A700-044), mouse anti-CD20 monoclonal [L26] (A500-017A). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P) and HRP-conjugated goat anti-mouse IgG (A90-116P). Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue).
Detection of human CD8 (red), FoxP3 (green), and PD-L1 (orange) in FFPE breast carcinoma by IHC-IF.
Detection of human CD3 (teal), CD8 (green), CD68 (orange), CK (red), Ki67 (yellow) and PD-L1 (white) in FFPE HNSCC by IHC-IF.
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Rabbit anti-CD3e recombinant monoclonal [BL-298-5D12] (A700-016), rabbit anti-CD8a recombinant monoclonal [BLR044F] (A700-044), mouse anti-CD68 monoclonal [KP-1] (A500-018A), mouse anti-cytokeratin monoclonal [AE1/AE3] (A500-019A), rabbit anti-Ki67 monoclonal [BLR021E] (A700-021) and rabbit anti-PD-L1 recombinant monoclonal [BLR020E] (A700-020). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P) and HRP-conjugated goat anti-mouse IgG (A90-116P). Substrate: Opal™ 480, 520, 570, 620, 690 and 780. Counterstain: DAPI (blue).
Ready to Start Multiplexing?
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