High-Performing ELISAs You Can Count On

Kits & Accessories

Detect your protein of interest with confidence using our ELISA kits. Every antibody that Bethyl sells has been manufactured and validated on-site to ensure high quality standards and reliable results. Select from an array of milk and serum proteins from many species and explore the assortment accessories – from solutions to buffers, and more – to support your most challenging research needs.

18 Different Milk and Serum Proteins

Albumin
Alpha-1-Antitrypsin
Hemoglobin
Hemoglobin F*
IgA
IgE

IgG
IgG1
IgG2
IgG2a
IgG2b
IgG2c

IgG3
IgM
Kappa
Lactoferrin
Lambda
Transferrin

* Hemoglobin F available custom by request

Across 9 Species

Bovine

Chicken

Dog

Goat

Human

Mouse

Pig

Rabbit

Rat

Find out if the ELISA technique is right for your research.

Enzyme linked immunosorbent assay, or ELISA, is a powerful qualitative and quantitative tool for measuring proteins in biologic samples. It can be used to compare differences in protein concentration between control and experimental groups in an in vivo or in vitro experiment, or between healthy donor and patient samples in clinical studies.

SARS-CoV-2 IgG ELISA Kit

Semi-quantitative

E88-301

SARS-CoV-2 IgM ELISA Kit

Semi-quantitative

E88-302

Bovine Albumin ELISA Kit

0.69 – 500 ng/ml

E11-113

Mouse Albumin ELISA Kit

1.23 – 900 ng/ml

E99-134

Human IgA ELISA Kit

1.03 – 750 ng/ml

E88-102

Human IgG ELISA Kit

0.69 – 500 ng/ml

E88-104

Human IgM ELISA Kit

1.03 – 750 ng/ml

E88-100

Pig IgG ELISA Kit

1.37 – 1000 ng/ml

E101-104

ELISA Kits

ELISA Kits include pre-coated 96-well strip plate, standard, detection antibody, sample dilution buffer, HRP solution, TMB substrate, stop solution, wash buffer, sealing tape and detailed instructions.

Description	Range	Catalog No.
Bovine Albumin ELISA Kit	0.69 – 500 ng/ml	E11-113
Bovine IgA ELISA Kit	1.37 – 1000 ng/ml	E11-121
Bovine IgG ELISA Kit	0.69 – 500 ng/ml	E11-118
Bovine IgG1 ELISA Kit	1.0 – 750 ng/ml	E11-116
Bovine IgG2 ELISA Kit	1.0 – 750 ng/ml	E11-117
Bovine IgM ELISA Kit	1.37 – 1000 ng/ml	E11-101
Bovine Lactoferrin ELISA Kit	0.69 – 500 ng/ml	E11-126
Chicken IgA ELISA Kit	1.37 – 1000 ng/ml	E33-103
Chicken IgG ELISA Kit	0.69 – 500 ng/ml	E33-104
Chicken IgM ELISA Kit	0.69 – 500 ng/ml	E33-102
Dog IgA ELISA Kit	1.37 –1000 ng/ml	E44-104
Dog IgG ELISA Kit	1.37 – 1000 ng/ml	E44-128
Goat IgG ELISA Kit	0.69 – 500 ng/ml	E55-104
Human Albumin ELISA Kit	0.69 – 500 ng/ml	E88-129
Human Alpha-1-Antitrypsin Kit	0.24 – 200 ng/ml	E88-122
Human Hemoglobin ELISA Kit	0.27 – 200 ng/ml	E88-134
Human Hemoglobin F ELISA Quantitation Set	6.25 - 400 ng/ml	E80-136
Human IgA ELISA Kit	1.03 – 750 ng/ml	E88-102
Human IgE ELISA Kit	0.69 – 500 ng/ml	E88-108
Human IgG ELISA Kit	0.69 – 500 ng/ml	E88-104
Human IgM ELISA Kit	1.03 – 750 ng/ml	E88-100
Human Kappa ELISA Kit	1.37 – 1000 ng/ml	E88-115
Human Lactoferrin ELISA Kit	1.56 – 100 ng/ml	E88-143
Human Lambda ELISA Kit	1.37 – 1000 ng/ml	E88-116
Human Transferrin ELISA Kit	0.69 – 500 ng/ml	E88-128
Mouse Albumin ELISA Kit	1.23 – 900 ng/ml	E99-134
Mouse IgA ELISA Kit	1.37 – 1000 ng/ml	E99-103
Mouse IgE ELISA Kit	1.37 – 1000 ng/ml	E99-115
Mouse IgG ELISA Kit	0.69 – 500 ng/ml	E99-131
Mouse IgG1 ELISA Kit	1.03 – 750 ng/ml	E99-105
Mouse IgG2a ELISA Kit	0.69 – 500 ng/ml	E99-107
Mouse IgG2b ELISA Kit	0.34 – 250 ng/ml	E99-109
Mouse IgG2c ELISA Kit	0.69 – 500 ng/ml	E99-136
Mouse IgG3 ELISA Kit	1.37 – 1000 ng/ml	E99-111
Mouse IgM ELISA Kit	1.37 – 1000 ng/ml	E99-101
Mouse Transferrin ELISA Kit	0.69 – 500 ng/ml	E99-129
Pig Albumin ELISA Kit	1.23 – 900 ng/ml	E101-110
Pig IgA ELISA Kit	1.37 – 1000 ng/ml	E101-102
Pig IgG ELISA Kit	1.37 – 1000 ng/ml	E101-104
Pig IgM ELISA Kit	1.37 – 1000 ng/ml	E101-117
Rabbit IgG ELISA Kit	0.69 – 500 ng/ml	E121-111
Rat Albumin ELISA Kit	1.37 – 1000 ng/ml	E111-125
Rat IgA ELISA Kit	1.37 – 1000 ng/ml	E111-102
Rat IgG ELISA Kit	1.37 – 1000 ng/ml	E111-128
Rat IgM ELISA Kit	2.74 – 2000 ng/ml	E111-100
SARS-CoV-2 IgG ELISA Kit	Semi-quantitative	E88-301
SARS-CoV-2 IgM ELISA Kit	Semi-quantitative	E88-302

ELISA Accessories

Description	Catalog #
ELISA Blocking Buffer	E104
ELISA Wash Solution	E106
ELISA Coating Buffer	E107
Microtiter Plate	E105
ELISA Starter Accessory Kit	E101
TMB Substrate	E102
ELISA Starter Accessory Kit II	E103
10% Tween 20	E108
ELISA Stop Solution	E115

ELISA Frequently Asked Questions

Can I use other ELISA buffers (ex. PBS) and substrates (ex. ABTS or OPD) with Bethyl’s ELISA kits?
Yes, our ELISA kits contain antibodies and a reference standard to establish a curve. We test and qualify our kits using the reagents that are listed in the product specification sheet. However, other standard ELISA reagents will work with our antibodies.

Do Bethyl’s ELISA kits contain polyclonal or monoclonal antibodies?
Polyclonal

What type of samples can I use with Bethyl’s ELISA kits?
Samples can be of biological (i.e. serum, plasma, urine, feces) or cell culture origin.

How should I store my samples before use?
We recommend that you store your samples at 4 C. However, biological or cell culture samples can be initially frozen until use but should not be repeatedly freeze-thawed. Once the sample is thawed, it should be stored at 4 C.

How do I dilute my samples?
Since all experimental designs are different, it is difficult to determine an overall dilution for all samples. If you have a general idea of the concentration of your samples, you will want to make dilutions so that your samples will fall in the middle range (or linear range) of the standard curve. Otherwise, you may want to take a few of your samples and make several dilutions (ex. 1:100, 1:1000, and 1:10,000) to determine a dilution range for your samples. Each investigator must determine the best dilution for their samples.

What if my raw OD values are too low or too high?
If the raw OD values appear too low, allow the reaction to continue by incubating the TMB development step longer than the 30 minutes outlined in our ELISA kit protocol. If the raw OD values appear to be developing too high, apply stop solution to the wells sooner than the 30 minute TMB incubation time.

What can I do to decrease my background?
Usually background is caused by cross contamination of samples or reagents. Make sure to always use a plate washer or wash by hand with a multi-channel pipette. Do not use the “squirt and dump” method for washing. You may also add another wash to each step. Make new reagents if background problems continue and make sure that you are not using a BSA product with an ELISA kit that may cross react (Bovine Albumin ELISA, Bovine Transferrin ELISA, Bovine IgG ELISA, Goat IgG ELISA, and others)

The quantitation of my sample is not consistent or seems to be incorrect. What can I do to correct this problem?
Make sure that you are quantitating your samples in the linear region of the curve. Samples that quantitate too close to the extreme ends of the curve may be inaccurate and difficult to reproduce.

Where can I buy the stop solution?
For your convenience, Bethyl Laboratories sells ready-to-use Stop Solution, catalog number E115.

What type of software is needed to graph a 4-parameter curve?
Some of the software choices are SoftMax Pro by Molecular Devices, KC Jr., SigmaPlot, or others. You cannot directly plot a 4-parameter curve with Microsoft EXCEL.

I do not have software that will perform a 4 parameter standard curve. What should I use to analyze my data?
You can use a linear regression curve in Microsoft EXCEL. If you use this type of curve, only use a maximum of 5 points on the curve. We recommend that you discard the upper and lower points and plot your standard curve with the 5 middle points.

We Make Every Antibody We Sell.

Bethyl is dedicated to delivering the highest standards of product and service quality. Recognizing that your research and time are valuable, we promise to stand behind our products. Every Bethyl product ships with a 100% guarantee; choose Bethyl antibodies with confidence.

Learn more about our validation process and our product guarantee.